Robbie Daymond

Peter Parker, Spider-Man, Superior Spider-Man

Nadji Jeter

Miles Morales, Security Robot, Miles Avatar

Fred Tatasciore

Max Modell, Hulk, Guard

Laura Bailey

Gwen Stacy, Ghost-Spider, Black Widow

Scott Menville

Otto Octavius, Grady Scraps, Superior Spider-Man

Melanie Minichino

Anya Corazon, Spider-Girl, Anya Avatar

Max Mittelman

Harry Osborn, Hobgoblin, Cop #2

Nancy Linari

Aunt May, Stage Manager, Bank Teller

Josh Keaton

Norman Osborn, John Jameson, Ollie Osnick

Bob Joles

J. Jonah Jameson, AIM Agent 1, Engineer

Ben Diskin

Flash Thompson, Spencer Smythe, Slayer

Alastair Duncan

Adrian Toomes, Vulture, Goblin King

John DiMaggio

Raymond Warren, The Jackal, Customer #1

Jason Spisak

Alistair Smythe, Scorpion, Audience Member

Katrina Kemp

Anna Maria, Student

Matthew Mercer

Aleksei Sytsevich, Rhino, Comic Book Guy #1

Yuri Lowenthal

Curt Connors, Lizard, Clash

Patton Oswalt

Uncle Ben, Chameleon