Nick Kroll

Nick Birch, Lola Skumpy, Maury the Hormone Monster

John Mulaney

Andrew Glouberman, Detective Florez, Mint

Jessi Klein

Jessi Glaser, Jessi Glaser as Margaret Daniels

Jason Mantzoukas

Jay Bilzerian, Guy Bilzerian, Sperm #1

Fred Armisen

Elliot Birch, Bus Driver, Ghost of Antonin Scalia

Maya Rudolph

Diane Birch, Connie the Hormone Monstress, Connie LaCienega

Jordan Peele

Ghost of Duke Ellington, Featuring Ludacris, Cyrus